---
author: einar
comments: true
date: 2006-11-10 22:04:54+00:00
layout: post
slug: the-joy-of-meta-analysis
title: The joy of meta-analysis
wordpress_id: 128
categories:
- Science
---

Recently, I've been in need to retrieve some records regarding renal cell carcinoma referenced in papers by [Zhao _et al._](http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&dopt=AbstractPlus&list_uids=16318415&query_hl=1&itool=pubmed_docsum) and[ Higgins _et al._  ](http://ajp.amjpathol.org/cgi/content/full/162/3/925)The records of the former were hosted on [NCBI's Gene Expression Omnibus](http://www.ncbi.nlm.nih.gov/geo), while the latter records were uploaded to EBI's [ArrayExpress](http://www.ebi.ac.uk/arrayexpress) database. Getting data from others and using it for your own analysis is called _meta-analysis_, and it's often used to validate methods and algorithms with different data sets.

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The problem is, getting the **right **data is not always easy. I spent the whole afternoon yesterday trying to figure out how I could retrieve already analyzed data (usually you get the processed - i.e. normalized - data only). From GEO I could download individual sample data (something I didn't need) or the whole data set (a whopping 1.6 Gb), in SOFTtext format. [Biopython](http://www.dennogumi.org/biopython.org) has a SOFT parser, but the set was so big I just crashed my own machine. Of course, data wasn't available in tabular format.

ArrayExpress wasn't better on that respect. Perhaps I don't understand well the format used by two color arrays, but again, it was impossible to group the samples like I wanted, and the sample information file was missing (critical requirement, I needed to choose only clear cell histotypes), though with some fiddling I managed to get the right files. Of course, they included only a normalized mean of the log2ratio of the two channels, and I didn't want to run an analysis (such as SAM) myself...

Science is all about being able to reproduce results. It's a shame that sometimes doing so is so hard.
